adiponectin globular recombinant protein  (MyBiosource Biotechnology)

Journal: Oncotarget

Article Title: Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth

doi: 10.18632/oncotarget.19905

Figure Lengend Snippet: Human and murine normal and pancreatic cancer samples were immunostained for ADIPOR1 and ADIPOR2. (A) strong staining is present in the acinar tissue of human normal samples for both receptors but is decreased in the human pancreatic tumor samples. (B) murine pancreatic cancer tissue samples from PKT mice demonstrate a high level of staining in the adjacent acinar tissue and a reduced staining in the dysplastic pancreatic tumor. Graphs represent quantitative analysis of mean fluorescence from single channel images. Scale bar is 100μm. (C-D) quantitative real time PCR analysis was used to determine the relative expression of AdipoR1 (white bars) and AdipoR2 (black bars) in human and murine pancreatic tumor samples (PDAC) as well as in pancreatic cancer cell lines, relative to the level expressed in the normal pancreas (NP) tissue. (E) quantitative real time PCR analysis for adiponectin expression in human and mouse pancreatic cancer cell lines and mouse tissue, relative to human adipose tissue (AT), 3T3L1 cells differentiated to adipocytes (3T3L1) and mouse AT, respectively. Statistical analysis was performed separately for ADIPOR1 and ADIPOR2 protein levels (unpaired t-test) as well as AdipoR1 and AdipoR2 expression (two-way Anova), or adiponectin expression (ordinary one-way Anova) (*P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001).

Article Snippet: Recombinant globular adiponectin (1688-AC or 1119-AC), full length adiponectin (1065-AP or 5095-AC), and Leptin (Mouse 498-OB or human 398-LP) from R&D System was used for in vitro analysis.

Techniques: Staining, Fluorescence, Real-time Polymerase Chain Reaction, Expressing

Journal: Oncotarget

Article Title: Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth

doi: 10.18632/oncotarget.19905

Figure Lengend Snippet: (A) recombinant globular adiponectin (gADN) at 1μg/mL or full length adiponectin (fADN) at 10μg/mL were incubated with human (Panc1 and MiaPaca2) and mouse (Panc02) pancreatic cancer cell lines, labeled with EdU and measured for active proliferation. DMSO was used as a control. Statistical analysis was performed using unpaired t-test (***P ≤ 0.001, ****P ≤ 0.0001). (B) human (Panc1 and MiaPaca2) and mouse PDAC cells (Panc02, P-4313 and K-8484) were treated with increasing concentration of AdipoRon for 48h before measuring proliferation via EdU, which showed dose-dependent inhibition for each PDAC cell line. Statistical significance was determined using ordinary one-way Anova (*P ≤ 0.05, ****P ≤ 0.0001).

Article Snippet: Recombinant globular adiponectin (1688-AC or 1119-AC), full length adiponectin (1065-AP or 5095-AC), and Leptin (Mouse 498-OB or human 398-LP) from R&D System was used for in vitro analysis.

Techniques: Recombinant, Incubation, Labeling, Control, Concentration Assay, Inhibition

Journal: Oncotarget

Article Title: Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth

doi: 10.18632/oncotarget.19905

Figure Lengend Snippet: Murine P-4313 and K-8484 cancer cells were orthotopically injected into syngeneic wildtype mice and allowed to grow for two weeks, after which mice were treated with vehicle or AdipoRon 5mg/kg/day for 2 weeks. (A) at endpoint, AdipoRon treated mice had a significantly reduced tumor size. (B) tumor proliferation measured by Ki67 positive tumor area per tumor section was significantly reduced after AdipoRon treatment. (C) no significant difference was detected in relative expression of AdipoR1 (white bars) and AdipoR2 (black bars) by quantitative real time PCR analysis between control and AdipoRon treated tumors. (D) ELISA analysis showed no significant difference in circulating serum adiponectin levels between the two groups of mice. (P-4313 filled symbols and K-8484 open symbols). Statistical analysis was performed using unpaired t-test (*P ≤ 0.05, **P ≤ 0.01).

Article Snippet: Recombinant globular adiponectin (1688-AC or 1119-AC), full length adiponectin (1065-AP or 5095-AC), and Leptin (Mouse 498-OB or human 398-LP) from R&D System was used for in vitro analysis.

Techniques: Injection, Expressing, Real-time Polymerase Chain Reaction, Control, Enzyme-linked Immunosorbent Assay

Journal: Endocrinology

Article Title: Central adiponectin acutely improves glucose tolerance in male mice.

doi: 10.1210/en.2013-1734

Figure Lengend Snippet: Figure 1. Gene expression of adiponectin, the receptor AdipoR , and the adaptor protein APPL1 in the ARC of either ad libitum or food- deprived mice. Adiponectin, AdipoR1, and APPL1 gene expression in the ARC is regulated by feeding status whereas AdipoR2 is unchanged. Autoradiographs depicting in situ hybridization with antisense 35S- labeled riboprobes to adiponectin (a), AdipoR1 (b), APPL1 (c), and AdipoR2 (d) mRNA signal in ad libitum-fed (upper left panels) and food-deprived (lower left panels) mice (n 6–7 mice per group). The right panels show a bar graph generated from quantification of the respective signal in the ARC. Means SEM of the quantified signal; **, P .01; ***, P .001

Article Snippet: In all experiments globular adiponectin was used (R&D Systems; catalog no. 1119-AC).

Techniques: Gene Expression, In Situ Hybridization, Labeling, Generated

Journal: Endocrinology

Article Title: Central adiponectin acutely improves glucose tolerance in male mice.

doi: 10.1210/en.2013-1734

Figure Lengend Snippet: Figure 3. Effect of centrally administered adiponectin on glucose tolerance in mice with genetically and/or diet-induced impaired energy, glucose metabolism, and pyruvate. Central adiponectin lowers peripheral glucose levels. a, ipGTT in Lepob/ob mice (n 9–11 mice per group) and respective wild-type controls (n 7–8 mice per group) that were either ICV injected with adiponectin (1 g in PBS) or vehicle (PBS). b, Pyruvate tolerance test of Lepob/ob mice and respective wild- type controls either ICV injected with adiponectin (1 g in PBS; n 5) or vehicle (PBS; n 6). c, ipGTTs of wild-type mice on LFD (ICV injected with vehicle [PBS; n 7]) and of wild-type mice fed HFD (60% fat; n 5 mice per group) ICV injected with either adiponectin (1 g in PBS) or vehicle (PBS). d, ipGTTs after either adiponectin (1 g in PBS; n 9) or vehicle (PBS; n 23) ICV injection in Lepob/ob mice on HFD (60% fat, 10 days). Adiponection and vehicle were injected 60 minutes prior to all tests. Shown are tolerance test (left panels) and respective AUCs (right panels). Data show means SEM; *, P .05; **, P .01; ***, P .001. ipPTT, ip pyruvate tolerance test; wt, wild type.

Article Snippet: In all experiments globular adiponectin was used (R&D Systems; catalog no. 1119-AC).

Techniques: Injection

Journal: Endocrinology

Article Title: Central adiponectin acutely improves glucose tolerance in male mice.

doi: 10.1210/en.2013-1734

Figure Lengend Snippet: Figure 4. Impact of centrally administered adiponectin on hypothalamic insulin and leptin- signaling pathways. Compared with vehicle-treated controls centrally administered adiponectin (1 g) partially increases the number of pAKT(Ser473) (a and b) and reduces the number of pAMPK(Thr172) (c and d) immunopositive cells in the ARC and VMH of Lepob/ob mice whereas pSTAT3(Tyr705) (e) immunoreactivity remains unaltered (n 5–6 mice per group). Mice received either adiponectin (1 g in PBS) or vehicle (PBS) 60 minutes prior to transcardial perfusion. Representative images show pAKT(Ser473), pAMPK(Thr172), and pSTAT3(Tyr705) immunoreactivity in the ARC and VMH. Immunoreactive cells counted in the respective region are shown in bar charts. Bar charts show means SEM; *, P .05; **, P .01

Article Snippet: In all experiments globular adiponectin was used (R&D Systems; catalog no. 1119-AC).

Techniques: Protein-Protein interactions

Journal: Endocrinology

Article Title: Central adiponectin acutely improves glucose tolerance in male mice.

doi: 10.1210/en.2013-1734

Figure Lengend Snippet: Figure 5. Central adiponectin reduces inflammatory signaling in the MBH during DIO. HF feeding (60% fat) increases the number of pJNK(Thr183/Tyr185) and reduces the number of pGSK3(Ser9) counted cells in the ARC and VMH of wild-type mice whereas central adiponectin (1 g in PBS) reverses this effect (n 5 mice per group). Wild-type mice were fed the HFD for 4 weeks. Mice received either adiponectin (1 g in PBS) or vehicle (PBS) 60 minutes prior to transcardial perfusion. Representative images show pJNK(Thr183/Tyr185) and pGSK3(Ser9) and immunoreactivity in the ARC and VMH. Immunoreactive cells counted in the respective region are shown in bar charts. Bar charts show means SEM; *, P .05; **, P .01; ***, P .001

Article Snippet: In all experiments globular adiponectin was used (R&D Systems; catalog no. 1119-AC).

Techniques:

Journal: PLoS ONE

Article Title: Drosophila Adiponectin Receptor in Insulin Producing Cells Regulates Glucose and Lipid Metabolism by Controlling Insulin Secretion

doi: 10.1371/journal.pone.0068641

Figure Lengend Snippet: (A) Amino acid sequence comparison between dAdipoR and human adiponectin receptor 1. Seven transmembrane domain regions are marked by upper lines. The putative interaction residues with the adiponectin are marked in blue. (B-I) Drosophila brains from the larva (B-E) and the adult (F-I) were immunostained with the dAdipoR antibody (green) and the IPCs marker, Dilp2>DsRed (red). dAdipoR staining was detected in IPCs (dot boxes), SOG neurons (arrows), and lateral neurons (arrowheads). Larval IPCs in B was enlarged in C-E and adult IPCs in F was enlarged in G-I, showing that dAdipoR staining in IPCs was overlapped with the IPCs marker. Scale bars are 100 µm (B, F) and 20 µm (E, I).

Article Snippet: The recombinant human globular adiponectin was purchased from R&D Systems.

Techniques: Sequencing, Comparison, Marker, Staining

Journal: PLoS ONE

Article Title: Drosophila Adiponectin Receptor in Insulin Producing Cells Regulates Glucose and Lipid Metabolism by Controlling Insulin Secretion

doi: 10.1371/journal.pone.0068641

Figure Lengend Snippet: (A) The dose effect of human adiponectin on Dilp2 secretion measured by Dilp2 staining intensity. 10 to 20 µg/ml of human adiponectin significantly induced Dilp2 secretion. (B-F) Images and relative intensities of Dilp2 staining in larval IPCs after treating with human adiponectin (10 µg/ml). DIlp2 secretion induced by human adiponectin was inhibited in IPCs of Dilp2>dAdipoR-Ri larvae. Data are presented as means ±SEM; **p<0.01. Scale bar is 20 µm (F).

Article Snippet: The recombinant human globular adiponectin was purchased from R&D Systems.

Techniques: Staining

Journal: Brain research

Article Title: AdipoR1 and 2 are expressed on warm sensitive neurons of the hypothalamic preoptic area and contribute to central hyperthermic effects of adiponectin

doi: 10.1016/j.brainres.2011.09.019

Figure Lengend Snippet: (A) Ethidium bromide stained agarose gel of amplification products of PCR carried out on cDNA from the POA or from POA single WSNs subject to aRNA amplification. 1 out of 8 WSN expressed AdipoR1 transcript; 2 expressed AdipoR2 (B-G) Representative immunohistochemistry of adiponectin receptor 1 (green) (arrow) (B) in the POA after retrograde transport tracing with Texas red (red) (arrow) (C) and nuclear staining with DAPI (blue) (arrow) (D). Images were merged (E) and reconstructed in three dimension at same (arrow) (F) or at higher magnification (G).

Article Snippet: Artificial cerebro-spinal fluid as vehicle, 12.5 ng mouse globular adiponectin (1119-Ac12, R&D, MN, USA) were injected directly to the target area through an implanted cannula using an injector (33 Ga, 10.5 mm length) connected to plastic tubing and a microsyringe in a volume of 0.5 μl over a period of 5 min to allow diffusion of the drugs.

Techniques: Staining, Agarose Gel Electrophoresis, Amplification, Immunohistochemistry

Journal: Brain research

Article Title: AdipoR1 and 2 are expressed on warm sensitive neurons of the hypothalamic preoptic area and contribute to central hyperthermic effects of adiponectin

doi: 10.1016/j.brainres.2011.09.019

Figure Lengend Snippet: 48 hrs profile of CBT recorded from wild type (A), AdipoR1 (B) null or AdipoR2 (C) null mice injected with adiponectin or aCSF used as vehicle (D) in the POA as indicated. Shaded area indicates dark part of the day. Injection was performed at time 4 hrs into the light part of the day (n= 6, p*<0.05).

Article Snippet: Artificial cerebro-spinal fluid as vehicle, 12.5 ng mouse globular adiponectin (1119-Ac12, R&D, MN, USA) were injected directly to the target area through an implanted cannula using an injector (33 Ga, 10.5 mm length) connected to plastic tubing and a microsyringe in a volume of 0.5 μl over a period of 5 min to allow diffusion of the drugs.

Techniques: Injection

Journal: Brain research

Article Title: AdipoR1 and 2 are expressed on warm sensitive neurons of the hypothalamic preoptic area and contribute to central hyperthermic effects of adiponectin

doi: 10.1016/j.brainres.2011.09.019

Figure Lengend Snippet: 48 hrs profile of motor activity recorded from wild type (A), AdipoR1 (B) null or AdipoR2 (C) null mice injected with adiponectin or aCSF used as vehicle (D) in the POAas indicated. Shaded area indicates dark part of the day. Injection was performed at time 4 hrs into the light part of the day (n= 6, p*<0.05).

Article Snippet: Artificial cerebro-spinal fluid as vehicle, 12.5 ng mouse globular adiponectin (1119-Ac12, R&D, MN, USA) were injected directly to the target area through an implanted cannula using an injector (33 Ga, 10.5 mm length) connected to plastic tubing and a microsyringe in a volume of 0.5 μl over a period of 5 min to allow diffusion of the drugs.

Techniques: Activity Assay, Injection

Journal: Brain research

Article Title: AdipoR1 and 2 are expressed on warm sensitive neurons of the hypothalamic preoptic area and contribute to central hyperthermic effects of adiponectin

doi: 10.1016/j.brainres.2011.09.019

Figure Lengend Snippet: 48 hrs profile of RER recorded from wild type (A), AdipoR1 (B) null or AdipoR2 (C) null mice injected with adiponectin or aCSF used as vehicle (D) in the POA as indicated. Shaded area indicates dark part of the day. Injection was performed at time 4 hrs into the light part of the day (n= 6, p*<0.05).

Article Snippet: Artificial cerebro-spinal fluid as vehicle, 12.5 ng mouse globular adiponectin (1119-Ac12, R&D, MN, USA) were injected directly to the target area through an implanted cannula using an injector (33 Ga, 10.5 mm length) connected to plastic tubing and a microsyringe in a volume of 0.5 μl over a period of 5 min to allow diffusion of the drugs.

Techniques: Injection